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نوع الوثيقة : مقال في مجلة دورية 
عنوان الوثيقة :
Flanking region variation of ForenSeq™ DNA signature prep kit STR and SNP loci in Yavapai Native Americans
Flanking region variation of ForenSeq™ DNA signature prep kit STR and SNP loci in Yavapai Native Americans
 
لغة الوثيقة : الانجليزية 
المستخلص : Massively parallel sequencing (MPS) offers advantages over current capillary electrophoresis-based analysis of short tandem repeat (STR) loci for human identification testing. In particular STR repeat motif sequence information can be obtained, thereby increasing the discrimination power of some loci. While sequence variation within the repeat region is observed relatively frequently in some of the commonly used STRs, there is an additional degree of variation found in the flanking regions adjacent to the repeat motif. Repeat motif and flanking region sequence variation have been described for major population groups, however, not for more isolated populations. Flanking region sequence variation in STR and single nucleotide polymorphism (SNP) loci in the Yavapai population was analyzed using the ForenSeqTM DNA Signature Prep Kit and STRait Razor v2s. Seven and 14 autosomal STRs and identity-informative single nucleotide polymorphisms (iiSNPs), respectively, had some degree of flanking region variation. Three and four of these identity-informative loci, respectively, showed _5% increase in expected heterozygosity. The combined length- and sequence-based random match probabilities (RMPs) for 27 autosomal STRs were 6.11 _10 _26 and 2.79 _ 10 _29, respectively. When combined with 94 iiSNPs (a subset of which became microhaplotypes) the combined RMP was 5.49 _ 10 _63. Analysis of length-based and sequence- based autosomal STRs in STRUCTURE indicated that the Yavapai are most similar to the Hispanic population. While producing minimal increase in X- and Y-STR discrimination potential, access to flanking region data enabled identification of one novel X-STR and three Y-STR alleles relative to previous reports. Five ancestry-informative SNPs (aiSNPs) and two phenotype-informative SNPs (piSNPs) exhibited notable flanking region variation. 
ردمد : 1872-4973 
اسم الدورية : Forensic Science International: Genetics 
المجلد : 28 
العدد : 1 
سنة النشر : 1438 هـ
2017 م 
نوع المقالة : مقالة علمية 
تاريخ الاضافة على الموقع : Thursday, June 1, 2017 

الباحثون

اسم الباحث (عربي)اسم الباحث (انجليزي)نوع الباحثالمرتبة العلميةالبريد الالكتروني
Wendt Frank RFrank R, Wendt باحث رئيسيدكتوراه 
King Jonathan LJonathan L, King باحثدكتوراه 
Novroski Nicole MMNicole MM, Novroski باحثدكتوراه 
Churchill Jennifer DJennifer D, Churchill باحثدكتوراه 
Ng JillianJillian, Ng باحثدكتوراه 
Oldt Robert FRobert F, Oldt باحثدكتوراه 
McCulloh Kelly LKelly L, McCulloh باحثدكتوراه 
Weise Jessica AJessica A, Weise باحثدكتوراه 
Smith David GlennDavid Glenn, Smith باحثدكتوراه 
Kanthaswamy SreetharanSreetharan, Kanthaswamy باحثدكتوراه 
Bruce BudowleBudowle, Bruce باحثدكتوراه 

الملفات

اسم الملفالنوعالوصف
 40839.pdf pdf 

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